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      1. 首页
      2. 设计载体
      3. 输基因,搜载体

      输基因,搜载体

      常见基因列表

      • Stuffer
      • Fluorescent Reporters
      • pH Sensitive Fluorescent Reporters
      • Chemiluminescent Reporters
      • Chromogenic Reporters
      • Selection Markers
      • Suicide Genes
      • Regulatory Proteins
      • Muscarinic Designer Receptors
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      • Recombinase
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      • Cas Proteins
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      • Pluripotency Genes
      名称 长度 描述
      ORF_Stuffer 249 bp Amino acid 2-83 of E. coli beta-galactosidase 指南 查看相关载体
      名称 长度 描述
      EGFP 720 bp Enhanced green fluorescent protein; codon optimized based on a variant of wild type GFP from the jellyfish Aequorea victoria 指南 查看相关载体
      NLS-EGFP 771 bp EGFP with nuclear localization signal on both ends 指南 查看相关载体
      sfGFP 717 bp Superfolder green fluorescent protein from Aequorea victoria; GFP variant S30R, Y39N, N105T, Y145F, I171V and A206V 指南 查看相关载体
      EmGFP 720 bp Emerald green fluorescent protein; variant of EGFP generated by mutagenesis 指南 查看相关载体
      TurboGFP 684 bp Also known as maxGFP; green fluorescent protein from maxillopoda 指南 查看相关载体
      hrGFP 720 bp Humanized recombinant green fluorescent protein derived from Renilla reniformis 指南 查看相关载体
      d2EGFP 846 bp Destabilized EGFP due to addition of PEST sequence from mouse Odc1 gene 指南 查看相关载体
      ZsGreen1 696 bp Bright green fluorescent protein derived from a Zoanthus sp. reef coral 指南 查看相关载体
      mNeonGreen 711 bp Yellow-green fluorescent protein derived from a tetrameric fluorescent protein from the cephalochordate Branchiostoma lanceolatum 指南 查看相关载体
      Venus 720 bp Variant of yellow fluorescent protein (YFP) generated by mutagenesis; YFP is itself a variant of EGFP generated by mutagenesis 指南 查看相关载体
      EYFP 720 bp Enhanced yellow fluorescent protein, GFP variant S65G/V68LS72A/T203Y 指南 查看相关载体
      YPet 720 bp Derived by combining mutations from Venus and YFP3; all these variants trace their origins to EGFP 指南 查看相关载体
      Cerulean 720 bp Improved ECFP 指南 查看相关载体
      CyPet 720 bp Variant of cyan fluorescent protein (CFP) generated by mutagenesis; CFP is itself a variant of EGFP generated by mutagenesis 指南 查看相关载体
      EBFP 720 bp Blue variant of EGFP generated by mutagenesis 指南 查看相关载体
      TagBFP 702 bp Also known as mTagBFP; blue variant of TagRFP generated by mutagenesis 指南 查看相关载体
      TagBFP2 702 bp Also known as mTagBFP2; improved variant of TagBFP generated by I174A mutation 指南 查看相关载体
      NLS_TagBFP2 753 bp TagBFP2 with nuclear localization signals on both ends 指南 查看相关载体
      dTomato 705 bp Dimeric variant of the Discosoma sp. red fluorescent protein (DsRed) generated by mutagenesis 指南 查看相关载体
      tdTomato 1431 bp Tandem dTomato generated by the fusion of two copies dTomato. 指南 查看相关载体
      NLS_tdTomato 1482 bp tdTomato with nuclear localization signals on both ends 指南 查看相关载体
      DsRed_Express2 678 bp Fast maturing variant of the Discosoma sp. red fluorescent protein (DsRed) generated by mutagenesis 指南 查看相关载体
      TurboRFP 696 bp Derived from the sea anemone Entacmaea quadricolor 指南 查看相关载体
      mRFP1 678 bp Monomeric red fluorescent protein 1; variant of DsRed generated by mutagenesis 指南 查看相关载体
      mCherry 711 bp Variant of mRFP1 generated by mutagenesis 指南 查看相关载体
      NLS_mCherry 762 bp mCherry with nuclear localization signals on both ends 指南 查看相关载体
      mApple 711 bp Photostable monomeric derivative of DsRed 指南 查看相关载体
      mKate2 699 bp Katushka2 far-red fluorescent protein 指南 查看相关载体
      名称 长度 描述
      pHluorin2 720 bp Enhanced, ratiometric, pH-sensitive green florescent protein 指南 查看相关载体
      Superecliptic-pHluorin 717 bp Red-shifted ecliptic pHluorin that is excited strongly by blue light at neutral pH but minimal fluorescence at acidic pH 指南 查看相关载体
      名称 长度 描述
      Luciferase 1653 bp Firefly luciferase 指南 查看相关载体
      Luc2 1653 bp Humanized firefly luciferase 指南 查看相关载体
      MetLuc 660 bp Metridia luciferase 指南 查看相关载体
      Rluc 936 bp Renilla luciferase 指南 查看相关载体
      hRluc 936 bp Humanized renilla luciferase 指南 查看相关载体
      Nluc 516 bp Nano Luciferase 指南 查看相关载体
      Aequorin 591 bp Calcium-activated photoprotein isolated from the hydromedusan Aequorea victoria 指南 查看相关载体
      名称 长度 描述
      LacZ 3051 bp E. coli beta-galactosidase 指南 查看相关载体
      SEAP 1560 bp Human secreted embryonic alkaline phosphatase 指南 查看相关载体
      名称 长度 描述
      Neo 795 bp Neomycin resistance gene 指南 查看相关载体
      Puro 600 bp Puromycin resistance gene 指南 查看相关载体
      Hygro 1026 bp Hygromycin resistance gene 指南 查看相关载体
      Bsd 399 bp Blasticidin resistance gene 指南 查看相关载体
      名称 长度 描述
      FKBP/Casp8 1227 bp FK506-binding protein 12 (with F36V mutation) fused with human caspase-8 protein deleted for 1-215 AA and linked to the N-myristoylation signal from Src kinase 指南 查看相关载体
      iCasp9 1242 bp FK506-binding protein 12 (with F36V mutation) fused with human caspase-9 protein deleted for 1-134 AA and linked to a HA tag 指南 查看相关载体
      deltaTK 996 bp Truncated thymidine kinase that converts ganciclovir (GCV) into a toxic metabolite that inhibits DNA synthesis 指南 查看相关载体
      CodA 1284 bp E.coli cytosine deaminase that catalyzes 5-fluorocytosine into toxic 5-fluorouracil 指南 查看相关载体
      DTA 657 bp Diphtheria toxin A 指南 查看相关载体
      DTR 627 bp Simian diphtheria toxin receptor 指南 查看相关载体
      名称 长度 描述
      MS2/P65/HSF1 1422 bp Fusion protein of MS2 bacteriophage coat protein, NF-kappaB trans-activating subunit p65 and human heat-shock factor 1 activation domain 指南 查看相关载体
      VP16 240 bp VP16 transcriptional activator domain encoded by HSV 指南 查看相关载体
      VP64 171 bp Tetrameric repeat of the minimal activation domain of VP16 指南 查看相关载体
      scFv(GCN4)-sfGFP-GB1-VP64 2031 bp Anti-GCN4 single chain variable fragment (scFv) antibody fused to superfolder-GFP (sfGFP) with a GB1 solubility tag and VP64 transcriptional activator domain 指南 查看相关载体
      KRAB 219 bp Krüppel-associated box domain from human gene ZNF10 指南 查看相关载体
      名称 长度 描述
      hM3D(Gq) 1791 bp A modified form of human Gq-coupled M3 muscarinic receptor, belonging to a class of chemogenetic receptors called designer receptors exclusively activated by designer drugs (DREADD) 指南 查看相关载体
      hM4D(Gi) 1458 bp A modified form of human Gi-coupled M4 muscarinic receptor, belonging to a class of chemogenetic receptors called designer receptors exclusively activated by designer drugs (DREADD) 指南 查看相关载体
      名称 长度 描述
      tTA 1008 bp Tetracycline transactivator 指南 查看相关载体
      tTA2 747 bp Tetracycline transactivator (2nd generation) 指南 查看相关载体
      rtTA 747 bp Reverse tetracycline responsive transcriptional activator M2 (2nd generation) 指南 查看相关载体
      tTS 849 bp Tetracycline transcriptional silencer 指南 查看相关载体
      tTS/rtTA 1656 bp tTS and rtTA_M2 linked by T2A 指南 查看相关载体
      Tet3G 747 bp Improved reverse tetracycline responsive transcriptional activator (3rd generation) 指南 查看相关载体
      名称 长度 描述
      ChR2 933 bp Human codon-optimized channelrhodopsin-2 from Chlamydomonas reinhardtii, a blue light-gated, cation-selective transmembrane channel protein. It rapidly undergoes conformational change upon absorbing photons to open channel permeable to cations (Na+, K+, H+, Ca2+, etc.) 指南 查看相关载体
      ChR2/mCherry 1647 bp ChR2 fused with mCherry by GCGGCCGCC 指南 查看相关载体
      ChR2/EYFP 1662 bp ChR2 fused with EYFP by GCGGCCGCC 指南 查看相关载体
      ChR2(H134R) 933 bp A gain-of-function mutant (H134R) of human codon-optimized channelrhodopsin-2 from Chlamydomonas reinhardtii 指南 查看相关载体
      ChR2(H134R)/mCherry 1647 bp ChR2(H134R) fused with mCherry by GCGGCCGCC 指南 查看相关载体
      ChR2(H134R)/EYFP 1662 bp ChR2(H134R) fused with EYFP by GCGGCCGCC 指南 查看相关载体
      eNpHR2.0/EYFP 1671 bp Enhanced Halorhodopsin from Natronomonas pharaonis (NpHR) which is fused with EYFP. NpHR is a fast light-activated electrogenic Cl- pump. There is an nAChR-derived signal peptide tagged to the N-terminal of NpHR, and a Kir2.1-derived ER export signal peptide tagged to the C-terminal of EYFP to facilitate cell surface expression of NpHR 指南 查看相关载体
      eNpHR3.0/EYFP 1680 bp Third generation of Halorhodopsin from Natronomonas pharaonis (NpHR) which is fused with EYFP. NpHR is a fast light-activated electrogenic Cl- pump. There is an nAChR-derived signal peptide tagged to the N-terminal of EYFP, and a Kir2.1-derived ER export signal peptide tagged to the C-terminal of EYFP to facilitate cell surface expression of NpHR 指南 查看相关载体
      eNpHR3.0/mCherry 1671 bp Third generation of Halorhodopsin from Natronomonas pharaonis (NpHR) which is fused with mCherry. NpHR is a fast light-activated electrogenic Cl- pump. There is an nAChR-derived signal peptide tagged to the N-terminal of mCherry, and a Kir2.1-derived ER export signal peptide tagged to the C-terminal of mCherry to facilitate cell surface expression of NpHR 指南 查看相关载体
      GCaMP6s 1353 bp Variant of green fluorescent calcium indicator GCaMP6 with slow kinetics, ultrasensitive sensor of free calcium 指南 查看相关载体
      GCaMP6m 1353 bp Variant of green fluorescent calcium indicator GCaMP6 with medium kinetics, ultrasensitive sensor of free calcium 指南 查看相关载体
      GCaMP6f 1353 bp Variant of green fluorescent calcium indicator GCaMP6 with fast kinetics, ultrasensitive sensor of free calcium 指南 查看相关载体
      jGCaMP7s 1353 bp Variant of the green fluorescent protein-based calcium indicator GCaMP7 with slow kinetics; ultrasensitive sensor of free calcium 指南 查看相关载体
      jGCaMP7f 1353 bp Variant of the green fluorescent protein-based calcium indicator GCaMP7 with fast kinetics; ultrasensitive sensor of free calcium 指南 查看相关载体
      jGCaMP7b 1353 bp Variant of the green fluorescent protein-based calcium indicator GCaMP7 with brighter baseline fluorescence; ultrasensitive sensor of free calcium 指南 查看相关载体
      jGCaMP7c 1353 bp Variant of the green fluorescent protein-based calcium indicator GCaMP7 which offers high contrast with low baseline fluorescence; ultrasensitive sensor of free calcium 指南 查看相关载体
      名称 长度 描述
      Cre 1056 bp Cyclization recombinase with SV40 nuclear localization signal 指南 查看相关载体
      InCre 1119 bp Cre with SV40 T-antigen intron inserted in the ORF 指南 查看相关载体
      Flpo 1299 bp Mouse codon-optimized version of Flpe recombinase with SV40 nuclear localization signal 指南 查看相关载体
      InFlpo 1365 bp Flpo with SV40 T-antigen intron inserted in the ORF 指南 查看相关载体
      Dre 1050 bp Dre Recombinase 指南 查看相关载体
      InDre 1116 bp Dre with SV40 T-antigen intron inserted in the ORF 指南 查看相关载体
      PBase 1785 bp Native piggyBac transposase from Trichoplusia ni 指南 查看相关载体
      hyPBase 1785 bp Hyperactive version of piggyBac transposase (PBase) created by mutagenesis 指南 查看相关载体
      hyPBase(R372A/K375A/D450N) 1785 bp Excision competent/integration defective piggyBac transposase with R372A, K375A and D450N mutations 指南 查看相关载体
      Tol2 1950 bp Transposase encoded from Tol2 transposon, a member of hAT transposable element family found in medaka fish genome 指南 查看相关载体
      SB100X 1023 bp Hyperactive variant of Sleeping Beauty transposase 指南 查看相关载体
      名称 长度 描述
      CreERT2 1983 bp Cyclization recombinase fused with ERT2 指南 查看相关载体
      DD-Cre 1533 bp Destabilized cyclization recombinase 指南 查看相关载体
      名称 长度 描述
      hCas9 4272 bp Human codon-optimized CRISPR associated protein 9 from Streptococcus pyogenes (with 3xFLAG tag and nuclear localization signal), also known as SpCas9 指南 查看相关载体
      Cas9(D10A) 4200 bp Human codon-optimized CRISPR associated protein 9 with D10A mutation, HA tag and nuclear localization signal 指南 查看相关载体
      dCas9 4107 bp Catalytically inactive Cas9, a variant of Cas9 bearing both D10A and H840A mutations 指南 查看相关载体
      dCas9/VP64 4395 bp dCas9 fused with a VP64 acidic transactivation domain 指南 查看相关载体
      dCas9/VPR 5799 bp dCas9 fused with a tripartite VP64-p65-Rta (VPR) transactivation domain 指南 查看相关载体
      dCas9/KRAB 4443 bp dCas9 fused with a KRAB transcriptional silencing domain 指南 查看相关载体
      dCas9/KRAB/MeCP2 5316 bp dCas9 fused with a carboxy terminal bipartite repressor domain, KRAB-MeCP2 指南 查看相关载体
      dCas9-10xGCN4_v4 5151 bp dCas9 with SV40 large T-antigen nuclear localization signals (nls) at both its amino and carboxyl termini, fused to an optimized GCN4 antibody-peptide pair (containing v4 version of the GCN4 peptide array with 10 copies of the peptide binding site, also know as SunTag10x_v4) 指南 查看相关载体
      SpCas9-HF1 4215 bp High-fidelity variant of SpCas9 指南 查看相关载体
      eSpCas9 4272 bp Enhanced specificity SpCas9 variant 指南 查看相关载体
      BE3 5133 bp rAPOBEC–XTEN–Cas9_D10A–UGI (rat cytidine deaminase enzyme fused with Cas9_D10A and uracil DNA glycosylase inhibitor via an XTEN linker) 指南 查看相关载体
      AsCpf1 4107 bp Also known as Cpf1; single RNA-guided endonuclease of a class 2 CRISPR-Cas system from Acidaminococcus, human codon-optimized, and linked to an HA tag and nuclear localization signal 指南 查看相关载体
      Cas9/mSA 4818 bp hCas9 fused with monomeric streptavidin 指南 查看相关载体
      SaCas9 3285 bp Staphylococcus aureus CRISPR associated protein 9 with HA tag and nuclear localization signal 指南 查看相关载体
      CjCas9 3012 bp A small Cas9 orthologue derived from Campylobacter jejuni with a carboxy terminal nuclear localization signal and HA tag 指南 查看相关载体
      名称 长度 描述
      SV40-T 2127 bp Simian virus 40 large T antigen 指南 查看相关载体
      hHRAS 513 bp Homo sapiens Ras proto-oncogene. Belongs to the Ras family of small GTPases, whose members are related to the transforming genes of mammalian sarcoma retroviruses 指南 查看相关载体
      hTERT 3399 bp Homo sapiens telomerase reverse transcriptase gene. Plays an improtant role in cellular senescence, and deregulation of telomerase expression in somatic cells may be involved in oncogenesis 指南 查看相关载体
      Ad5E1A 732 bp Human adenovirus type 5 E1A protein gene 指南 查看相关载体
      HPV16E6 477 bp Human papillomavirus type 16 transforming protein E6 gene 指南 查看相关载体
      HPV16E7 297 bp Human papillomavirus type 16 transforming protein E7 gene 指南 查看相关载体
      hTP53(V143A) 1182 bp Homo sapiens tumor suppressor p53 gene with a V143A mutation 指南 查看相关载体
      名称 长度 描述
      hMYC 1365 bp Homo sapiens c-myc proto-oncogene. Belongs to myelocytomatosis (Myc) family of transcription factors. Plays a role in cell cycle procession, apotosis and cellular transformation 指南 查看相关载体
      hOct4 1083 bp Homo sapiens Oct-4 gene. Encodes a transcription factor containing a POU homeodomain that plays a key role in embryonic development and stem cell pluripotency. Aberrant expression in adult tissues is associated with tumorigenesis. 指南 查看相关载体
      hSox2 954 bp Homo sapiens transcription factor SOX-2 gene. Belongs to the SRY-related HMG-box (SOX) family of transcription factors, which is involved in the regulation of embryonic development and in the determination of cell fate 指南 查看相关载体
      mMyc 1365 bp Mus musculus myc proto-oncogene. Belongs to myelocytomatosis (Myc) family of transcription factors. Plays a role in cell cycle procession, apotosis and cellular transformation 指南 查看相关载体
      mOct4 1059 bp Mus musculus Oct-4 gene. Encodes a transcription factor containing a POU homeodomain that plays a key role in embryonic development and stem cell pluripotency. 指南 查看相关载体
      mSox2 960 bp Mus musculus transcription factor SOX-2 gene. Belongs to the SRY-related HMG-box (SOX) family of transcription factors, which is involved in the regulation of embryonic development and in the determination of cell fate 指南 查看相关载体
      hNanog 918 bp Homo sapiens nanog homeobox gene. Encodes a DNA-binding homeobox-family transcription factor involved in embryonic stem (ES) cell proliferation, renewal, and pluripotency. 指南 查看相关载体
      mNanog 918 bp Mus musculus nanog homeobox gene. Encodes a DNA-binding homeobox-family transcription factor involved in embryonic stem (ES) cell proliferation, renewal, and pluripotency. 指南 查看相关载体
      hKlf4 1440 bp Homo sapiens Kruppel-like factor 4 (Klf4) gene. Belongs to the relatively large family of SP1-like transcription factors and is involved in the regulation of proliferation, differentiation, apoptosis and somatic cell reprogramming. 指南 查看相关载体
      mKlf4 1452 bp Mouse Kruppel-like factor (Klf4) gene. Belongs to the relatively large family of SP1-like transcription factors and is involved in the regulation of proliferation, differentiation, apoptosis and somatic cell reprogramming. 指南 查看相关载体
      hLin28 630 bp Human lin-28 homolog A gene. Encodes a LIN-28 family RNA-binding protein that acts as a posttranscriptional regulator of genes involved in development, self-renewal of embryonic stem cells and metabolism. Overexpressed in human embryonic stem cells, primary human tumors and human cancer cell lines. 指南 查看相关载体
      mLin28 630 bp Mouse lin-28 homolog A gene. Encodes a LIN-28 family RNA-binding protein that acts as a posttranscriptional regulator of genes involved in development, self-renewal of embryonic stem cells and metabolism. Highly expressed in mouse embryonic stem cells and during early embryogenesis. 指南 查看相关载体
      hEsrrb 1527 bp Human estrogen-related-receptor β gene. Encodes a member of the nuclear orphan receptor family that is involved in early development, pluripotency and reprogramming. 指南 查看相关载体
      mEsrrb 1365 bp Mouse estrogen-related-receptor β gene. Encodes a member of the nuclear orphan receptor family that is involved in early development, pluripotency and reprogramming. 指南 查看相关载体
      mOSKM 5064 bp A combination of four reprogramming factor genes (mouse Oct3/4, Sox2, Klf4 and c‐Myc) linked by three 2A “self-cleaving” peptides. Involved in reprogramming, or generation of induced pluripotent stem (iPS) cells. Their expression commonly increases in metastatic cancers. 指南 查看相关载体
      hOSKM 5070 bp A combination of four reprogramming factor genes (human Oct4, Sox2, Klf4 and c‐Myc) linked by three 2A “self-cleaving” peptides. Involved in reprogramming, or generation of induced pluripotent stem (iPS) cells. Their expression commonly increases in metastatic cancers. 指南 查看相关载体
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