eNpHR2.0/EYFP 载体

基因信息

基因名称eNpHR2.0/EYFP
类型ORF
子类Optogenetic protein
描述Enhanced Halorhodopsin from Natronomonas pharaonis (NpHR) which is fused with EYFP. NpHR is a fast light-activated electrogenic Cl- pump. There is an nAChR-derived signal peptide tagged to the N-terminal of NpHR, and a Kir2.1-derived ER export signal peptide tagged to the C-terminal of EYFP to facilitate cell surface expression of NpHR
应用注释Light activation of NpHR leads to cell hyperpolarization and inhibition of firing action potentials; increased peak photocurrent compared to original NpHR; no aggregation; low cellular toxicity; maximum excitation is 580 nm; maximum excitation and emission of EYFP are 513 nm and 527 nm, respectively.
参考文献Nature. 446:633 (2007); Brain Cell Biol. 36:129 (2008)
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载体描述	载体类型	标记基因
eNpHR2.0/EYFP expression regular plasmid 载体	Mammalian Gene Expression Vector 指南
eNpHR2.0/EYFP expression lentivirus 载体	Mammalian Gene Expression Lentiviral Vector 指南
eNpHR2.0/EYFP expression adenovirus 载体	Mammalian Gene Expression Adenoviral Vector 指南
eNpHR2.0/EYFP expression AAV病毒载体载体	Mammalian Gene Expression AAV Vector 指南	--
eNpHR2.0/EYFP expression PiggyBac转座载体载体	Mammalian Gene Expression PiggyBac Vector 指南