相关服务
载体构建质粒DNA制备
病毒包装服务
mRNA基因递送解决方案
CRISPR基因编辑解决方案
shRNA基因敲低解决方案
Molarity is defined as the number of moles of solute dissolved in one liter of solution and is
expressed in units of moles per liter (M). A stock solution is a concentrated solution prepared to a
high molarity. Researchers often prepare stock solutions to conserve space and resources, as these
concentrated solutions can be stored for long periods and diluted as needed to make working
solutions.
The formula 𝐶1𝑉1 = 𝐶2𝑉2 can be
used to determine the volume of stock
solution needed to
achieve a desired final concentration and volume of a working solution. Where 𝐶1 is the
stock
concentration, 𝑉1 is the volume of the stock solution required, 𝐶2 is the
desired concentration,
and 𝑉2 is the final volume of the working solution. This equation can be arranged as
below to
determine the amount of stock solution required to prepare a working solution at a given volume and
concentration.
Figure 1. Calculation of the amount of stock solution needed for a working solution at a desired concentration and volume.
Serial dilution is a step-wise dilution of a solution by a select factor. Serial dilutions are used for analytical purposes and in addition are a method to easily dilute a highly concentrated solution. Most serial dilutions are performed by a dilution factor of 10; for instance, 100 ul of stock solution is added to 900 ul of solvent followed by mixing and subsequent repeats of this dilution until the desired molarity is achieved.
Figure 2. Example preparation of a serial dilution with a dilution factor of 10.
To calculate the weight of a substance needed to prepare a stock solution or a working solution directly, use VectorBuilder’s Molarity Calculator.