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Accurately measuring the quantity of your protein is imperative for a range of molecular biology
applications, including recombinant protein expression, western blotting, transfection, and enzymatic
assays. VectorBuilder’s protein calculator allows you to easily retrieve molecular weight and amount of
your protein (mass or moles) to ensure correct setup of your experiment.
Molecular weight calculation
Knowing the molecular weight of your compound is important for biology applications ranging from simple molecular biology processes like western blotting to clinical drug development. Molecular weight is determined by summing the weights of the protons, neutrons, and electrons in a molecule. To convert between mass and moles of a biomolecule, the molecular weight of a biomolecule must first be obtained. To determine the molecular weight of a protein, sum the molecular weights of each amino acid in the protein. Example calculations are shown below for amino acid serine. Alternatively, if just the sequence length is entered, the average amino acid molecular weight is multiplied by the length for an approximation.
Figure 1. Chemical structure of serine and example calculation of its molecular weight.
Mass - moles converter
In chemistry, a mole represents a quantity of substance containing Avogadro's number of particles (6.02214076 × 1023), typically atoms or molecules. After obtaining the molecular weight of a biomolecule by summing the molecular weights of its components, the mass and moles of a substance can be calculated using the following equation:
Mass Protein (g) = Moles Protein (mol) x Molecular Weight (g/mol)
Figure 2. Equation used to convert between moles and mass of a substance with known molecular weight.
