Popular Vectors for Gene (mt-mKeima)

基因信息

基因名称mt-mKeima
类型CDS
子类pH-Sensitive Fluorescent Reporters
描述Monomeric dual-excitation Keima protein derived from stony coral Montipora by numerous cycles of semi-random mutagenesis; fused with a tandem repeat of mitochondria-targeting sequences from COX VIII
应用注释Localizes to the mitochondrial matrix for the detection of mitophagy using reporter color change. The excitation wavelength of the mKeima fluorescent protein is determined by the pH of the environment, with an excitation wavelength centered at 440 nm at neutral pH and an excitation of 586 nm at an acidic pH. The emission wavelength of Keima is identical at either pH and peaks maximally at 620 nm.
参考文献Chem Biol.18:1042 (2011); Nat Protoc.12:1576 (2017)
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载体描述	载体类型	标记基因
mt-mKeima expression regular plasmid 载体	Mammalian Gene Expression Vector 指南
mt-mKeima expression lentivirus 载体	Mammalian Gene Expression Lentiviral Vector 指南
mt-mKeima expression adenovirus 载体	Mammalian Gene Expression Adenoviral Vector 指南
mt-mKeima expression AAV病毒载体载体	Mammalian Gene Expression AAV Vector 指南	--
mt-mKeima expression PiggyBac转座载体载体	Mammalian Gene Expression PiggyBac Vector 指南

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