元件指南

VectorBuilder提供了许多常用的载体元件，可供用户在设计载体时进行选择。下表按类型提供了这些常用元件的详细信息。

姓名	靶序列	Target Gene	Target Position	References	序列
Scramble[shRNA#1]	CCTAAGGTTAAGTCGCCCTCG	None in human and mouse		Cancer Res. 66:9270 (2006)	View
Scramble[shRNA#2]	CAACAAGATGAAGAGCACCAA	TurboGFP; none in human and mouse		Oncogene. 30:1551 (2011); Science. 343:309 (2014)	View
EGFP[shRNA#1]	CGACGTAAACGGCCACAAGTT	EGFP	63-83	The RNAi Consortium ID: TRCN0000072193	View
EGFP[shRNA#2]	ACGTCTATATCATGGCCGACA	EGFP	449-469	The RNAi Consortium ID: TRCN0000231756	View
LacZ[shRNA#1]	GTCGGCTTACGGCGGTGATTT	LacZ	1734-1754	The RNAi Consortium ID: TRCN0000072242	View
LacZ[shRNA#2]	GTTCCGTCATAGCGATAACGA	LacZ	1908-1928	The RNAi Consortium ID: TRCN0000072238	View
Luciferase[shRNA#1]	ATGTTTACTACACTCGGATAT	Firefly luciferase	745-765	The RNAi Consortium ID: TRCN0000231737	View
Luciferase[shRNA#2]	TGTCCGGTTATGTAAACAATC	Firefly luciferase	1193-1213	The RNAi Consortium ID: TRCN0000072258	View
Scramble[miR30-shRNA#1]	ACCTAAGGTTAAGTCGCCCTCG	None in human and mouse		Designed by VectorBuilder	View
Scramble[miR30-shRNA#2]	CCAACAAGATGAAGAGCACCAA	TurboGFP; none in human and mouse		Oncogene. 30:1551 (2011); Science. 343:309 (2014)	View
EGFP[miR30-shRNA#1]	GCGACGTAAACGGCCACAAGTT	EGFP	62-83	Designed by VectorBuilder	View
EGFP[miR30-shRNA#2]	AACGTCTATATCATGGCCGACA	EGFP	448-469	Designed by VectorBuilder	View
EGFP[miR30-shRNA#3]	AAGAACGGCATCAAGGTGAACT	EGFP	475-496	Designed by VectorBuilder	View
EGFP[miR30-shRNA#4]	CACATGAAGCAGCACGACTTCT	EGFP	232-253	Designed by VectorBuilder	View