元件指南

VectorBuilder提供了许多常用的载体元件，可供用户在设计载体时进行选择。下表按类型提供了这些常用元件的详细信息。
Regulatory Elements
姓名描述Application NotesReferences序列
WPREWoodchuck hepatitis virus posttranscriptional regulatory element Enhances virus stability in packaging cells, leading to higher titer of packaged virus; enhances higher expression of transgenes.J Virol. 73:2886 (1999); Hum Gene Ther. 10:2295 (1999) View
WPRE3Truncated short version of woodchuck hepatitis virus posttranscriptional regulatory element (248 bp)Contained only minimal gamma and alpha subunits of WPRE. Slightly weaker effective compared to WPRE.Mol Brain. 7:17(2014); Nat Biomed Eng. 6:1272 (2022) View
oPREOptimized and truncated version of woodchuck hepatitis virus posttranscriptional regulatory elementThe potentially oncogenic X protein ORF and X protein promoter have been removed; ATG start sites in ORFs encoding sequences greater than 25 amino acids in length have been mutated.Gene Ther. 13:641 (2006); Hum Gene Ther Methods. 23: 73 (2012) View