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When a
transgenic animal carrying such a vector is crossed to an animal carrying a
tissue-specific Cre transgene, the gene of interest would be turned off in the
progeny animals carrying both types of transgenes, specifically in cells where
the tissue-specific Cre is expressed and the user-selected promoter driving
the gene of interest is active.
For further information about this vector system and Cre-mediated
recombination, please refer to the papers below....
Structurally, a CAR consists of four main components: (1) an extracellular
antigen recognition domain made up of an antibody-derived single chain
variable fragment (scFv) of known specificity....
Structurally, a CAR consists of four main components: (1) an extracellular
antigen recognition domain made up of an antibody-derived single chain
variable fragment (scFv) of known specificity....
Structurally, a CAR consists of four main components: (1) an extracellular
antigen recognition domain made up of an antibody-derived single chain
variable fragment (scFv) of known specificity....
Transgenic
animals carrying such a vector originally express the first user-selected ORF,
however when crossed to an animal carrying a tissue-specific Cre transgene,
expression of the second user-selected ORF will be activated while silencing
the first ORF in the progeny animals carrying both types of transgenes,
specifically in cells where the tissue-specific Cre is expressed and the user-
selected promoter is active....