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When a transgenic animal carrying such a vector is crossed to an animal carrying a tissue-specific Cre transgene, the gene of interest would be turned off in the progeny animals carrying both types of transgenes, specifically in cells where the tissue-specific Cre is expressed and the user-selected promoter driving the gene of interest is active. For further information about this vector system and Cre-mediated recombination, please refer to the papers below....
Structurally, a CAR consists of four main components: (1) an extracellular antigen recognition domain made up of an antibody-derived single chain variable fragment (scFv) of known specificity....
Structurally, a CAR consists of four main components: (1) an extracellular antigen recognition domain made up of an antibody-derived single chain variable fragment (scFv) of known specificity....
Structurally, a CAR consists of four main components: (1) an extracellular antigen recognition domain made up of an antibody-derived single chain variable fragment (scFv) of known specificity....
Transgenic animals carrying such a vector originally express the first user-selected ORF, however when crossed to an animal carrying a tissue-specific Cre transgene, expression of the second user-selected ORF will be activated while silencing the first ORF in the progeny animals carrying both types of transgenes, specifically in cells where the tissue-specific Cre is expressed and the user- selected promoter is active....
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