搜索“微载体细胞培养榜篯端游捏脸吗 sitezhidaobaiducom”得到76个结果，以下为结果71-76
## 概述
CRISPR/Cas9载体属于几种新兴的基因组编辑工具之一，可以快速有效地在基因组的靶位点产生突变（另外两种应用广泛的是ZFN和TALEN）。
Cas9属于RNA引导的DNA核酸酶，是天然原核免疫系统的一部分，赋予细菌对质粒和噬菌体等外源遗传物质的抗性。在细胞内，Cas9核酸酶与引导RNA（gRNA）形成复合物，该复合物通过与基因组中的18-22
nt的同源靶序列直接相互作用提供靶向特异性。gRNA与靶位点通过互补配对使Cas9定位到靶序列上，然后切割基因组中的靶位点。
协同激活介质（SAM）系统是用于转录激活内源性基因的强大工具。该系统源自CRISPR/Cas9基因组编辑系统，但不具有基因组编辑功能，是一种可指导在靶位点进行多组分转录激活复合物（SAM复合物）组装的修饰型gRNA。通常，SAM复合物的组装足以诱导靶位点强烈的转录激活。
展开 >>
完整的SAM系统包含三个组分，每个组分分别由单独的慢病毒载体提供：gRNA/MS2表达载体，MS2/P65/HSF1和dCas9/VP64辅助载体。
将用户定制的gRNA序列克隆到gRNA/MS表达载体。...
## 概述
该载体系统设计用于在小鼠模型中，高效分析哺乳动物增强子。通常情况下，将感兴趣的增强子克隆到该载体中，并将构建好的载体用于制备转基因小鼠。对转基因胚胎或成年小鼠中LacZ报告基因的表达情况进行检测，可以进一步分析增强子的活性。
该载体系统可用于筛选增强子元件，分析增强子的组织特异性，比较不同增强子的活性，谱系追踪以及其他应用。
关于该载体系统的更多信息，请参考一下文献。
参考文献 | 主题
---|---
Nature. 444:499-502 (2006) | Use of the vector systemtocarry out genome-wide testing of putative enhancers in the mouse
Development. 105:707-714 (1989) | Cloning of the Hsp68 minimal promoter
## 亮点
我们的载体系统是基于常规质粒基因表达载体而构建的。待测增强子位于Hsp68最小启动子（Hsp68_mini）的上游，可控制下游LacZ报道基因的表达。...
#### Overview
The RCAS (Replication-Competent Avian Sarcoma-Leukosis Virus long terminal
repeat with a Splice acceptor) retroviral vectors are derived from the Rous
sarcoma virus (RSV). Wildtype RSV retains all essential viral genes and the
oncogene src. In RCAS vectors, the src oncogene is deleted and replaced with a
transgene of interest while maintaining the src splice sites, enabling
expression of the inserted gene from a spliced viral transcript driven by the
viral long terminal repeat (LTR...
.
## 载体关键元件
Ylp (Integrating plasmid)
YEp (Episomal plasmid)
YCp (Centromere plasmid)
Promoter: The promoter that drives your gene of interest is placed here. When
the inducible GAL1 promoter is used, galactose will induce high-level
transcription of the gene of interest, while glucose will strongly repress
expression.
Kozak: Kozak consensus sequence. It is placed in front of the start codon of
the ORF of interest because it is believed to facilitate translation
initiation in eukaryotes....
.
#### 载体关键元件
CMV promoter: Human cytomegalovirus immediate early promoter. It drives
transcription of viral RNA in packaging cells. This RNA is then packaged into
live virus.
MMLV 5' LTR- ΔU3: A deleted version of the MMLV retrovirus 5' long terminal
repeat. In wildtype MMLV retrovirus, 5' LTR and 3' LTR are essentially
identical in sequence. They reside on two ends of the viral genome and point
in the same direction. Upon viral integration, the 3' LTR sequence is copied
onto the 5' LTR....
.
#### 载体关键元件
CMV promoter: Human cytomegalovirus immediate early promoter. It drives
transcription of viral RNA in packaging cells. This RNA is then packaged into
live virus.
MMLV 5' LTR- ΔU3: A deleted version of the MMLV retrovirus 5' long terminal
repeat. In wildtype MMLV retrovirus, 5' LTR and 3' LTR are essentially
identical in sequence. They reside on two ends of the viral genome and point
in the same direction. Upon viral integration, the 3' LTR sequence is copied
onto the 5' LTR....
服务商工作站 >>
