搜索“win11专where 一样送到菜鸟苷酸结合蛋白1”得到106个结果，以下为结果91-100
It utilizes adenovirus-mediated delivery of a
polycistronic expression cassette consisting of one or more miR30-based shRNAs
(shRNAmiR) targeting gene(s) of interest and a user-selected ORF, where the
vector remains as episomal DNA without integration into the host genome. The
shRNAmiR transcript is processed by endogenous, cellular micro-RNA pathways to
produce mature shRNAs, which facilitate degradation of target gene mRNAs....
It is then
transfected into packaging cells along with helper plasmids, where the region
of the vector between the two inverted terminal repeats (ITRs) is packaged
into live virus. For the AAV (FLEX) conditional Cre-On gene expression vector,
the FLEX Cre-On switch described above is placed in-between the two ITRs
during vector construction, which is introduced into target cells along with
the rest of viral genome....
It is then
transfected into packaging cells along with helper plasmids, where the region
of the vector between the two inverted terminal repeats (ITRs) is packaged
into live virus. Any gene(s) placed in-between the two ITRs are introduced
into target cells along with the rest of viral genome.
The wild-type AAV genome is a linear single-stranded DNA (ssDNA) with two ITRs
forming a hairpin structure on each end. It is therefore also known as ssAAV....
It is then
transfected into packaging cells along with helper plasmids, where the region
of the vector between the two inverted terminal repeats (ITRs) is packaged
into live virus. For the AAV (FLEX) conditional Cre-Off gene expression
vector, the FLEX Cre-Off switch described above is placed in-between the two
ITRs during vector construction, which is introduced into target cells along
with the rest of viral genome....
When the virus is
added to target cells, the RNA cargo is shuttled into cells where it is
reverse transcribed into DNA and permanently integrated into the host genome,
leading to the expression of the user-selected gRNA sequence.
Our lentivirus gRNA expression vector is available for expressing either
single-gRNA or dual-gRNAs....
A key feature of
transfection with regular plasmid vectors is that it is transient, with only a
very low fraction of cells stably integrating the plasmid in the genome
(typically less than 1%).
For further information about this vector system, please refer to the papers
below....
When the virus is added to target cells, the RNA cargo is
shuttled into cells where it is reverse transcribed into DNA and permanently
integrated into the host genome, leading to the co-expression of Cas9 and the
user-selected gRNA sequence.
Our lentivirus CRISPR vector is available for expressing either single-gRNA or
dual-gRNAs....
A key feature of transfection with regular plasmid
vectors is that it is transient, with only a very low fraction of cells stably
integrating the plasmid in the genome (typically less than 1%).
Our regular plasmid gRNA expression vector is available for expressing either
single-gRNA or dual-gRNAs....
Additionally, the AAV msSagRNA vector drives the expression of a three-domain
fusion protein consisting of MS2, p65 (the trans-activation subunit of NF-kB),
and HSF1 (the activation domain of human heat shock factor 1). The AAV
dSaCas9/VP64 helper vector on the other hand drives the expression of a fusion
protein consisting of a catalytically inactive variant of SaCas9 and the
synthetic VP64 transactivation domain....
When a
transgenic animal carrying such a vector is crossed to an animal carrying a
tissue-specific Cre transgene, the gene of interest would be turned off in the
progeny animals carrying both types of transgenes, specifically in cells where
the tissue-specific Cre is expressed and the user-selected promoter driving
the gene of interest is active.
For further information about this vector system and Cre-mediated
recombination, please refer to the papers below....
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