搜索“win11专where 一样送到菜鸟苷酸结合蛋白1”得到105个结果，以下为结果91-100
It utilizes adenovirus-mediated delivery of a
polycistronic expression cassette consisting of one or more miR30-based shRNAs
(shRNAmiR) targeting gene(s) of interest and a user-selected ORF, where the
vector remains as episomal DNA without integration into the host genome. The
shRNAmiR transcript is processed by endogenous, cellular micro-RNA pathways to
produce mature shRNAs, which facilitate degradation of target gene mRNAs....
It is then
transfected into packaging cells along with helper plasmids, where the region
of the vector between the two inverted terminal repeats (ITRs) is packaged
into live virus. For the AAV (FLEX) conditional Cre-On gene expression vector,
the FLEX Cre-On switch described above is placed in-between the two ITRs
during vector construction, which is introduced into target cells along with
the rest of viral genome....
It is then
transfected into packaging cells along with helper plasmids, where the region
of the vector between the two inverted terminal repeats (ITRs) is packaged
into live virus. For the AAV (FLEX) conditional Cre-Off gene expression
vector, the FLEX Cre-Off switch described above is placed in-between the two
ITRs during vector construction, which is introduced into target cells along
with the rest of viral genome....
This is particularly powerful in
avian embryo models, where localized infection enables spatially and
temporally controlled gene expression for the in vivo study of developmental
processes such as neural patterning and limb development.
In mammalian systems, RCASBP(A) vectors are widely used in combination with
TVA-expressing cells or transgenic mouse models to achieve highly targeted
gene delivery....
When the virus is
added to target cells, the RNA cargo is shuttled into cells where it is
reverse transcribed into DNA and permanently integrated into the host genome,
leading to the expression of the user-selected gRNA sequence.
Our lentivirus gRNA expression vector is available for expressing either
single-gRNA or dual-gRNAs....
A key feature of
transfection with regular plasmid vectors is that it is transient, with only a
very low fraction of cells stably integrating the plasmid in the genome
(typically less than 1%).
For further information about this vector system, please refer to the papers
below....
When the virus is added to target cells, the RNA cargo is
shuttled into cells where it is reverse transcribed into DNA and permanently
integrated into the host genome, leading to the co-expression of Cas9 and the
user-selected gRNA sequence.
Our lentivirus CRISPR vector is available for expressing either single-gRNA or
dual-gRNAs....
A key feature of transfection with regular plasmid
vectors is that it is transient, with only a very low fraction of cells stably
integrating the plasmid in the genome (typically less than 1%).
Our regular plasmid gRNA expression vector is available for expressing either
single-gRNA or dual-gRNAs....
Additionally, the AAV msSagRNA vector drives the expression of a three-domain
fusion protein consisting of MS2, p65 (the trans-activation subunit of NF-kB),
and HSF1 (the activation domain of human heat shock factor 1). The AAV
dSaCas9/VP64 helper vector on the other hand drives the expression of a fusion
protein consisting of a catalytically inactive variant of SaCas9 and the
synthetic VP64 transactivation domain....
Examples of such
applications include: 1) paired Cas9 nickase experiments where the "nickase"
mutant form (hCas9-D10A) of hCas9 is used in conjunction with two gRNAs
targeting the two opposite strands of a single target site to generate single
strand cuts one on each strand, thereby leading to a DSB with increased
targeting specificity than a single gRNA; 2) generating deletion of a fragment
between two DSBs targeted by a pair of gRNAs; and 3) targeting two different
genes simultaneously....
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