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关键词: CAR-T cell therapy, Kymriah CAR-T, immunotherpy
A deep dive into the biology behind CAR-T vectors, their clinical applications, and how CRISPR/Cas9 is being used to create the next generation of CAR therapeutics.
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关键词: Cell culture media, plasmid yield, bacterial growth
Plasmid yield from bacterial cultures can be enhanced by optimizing antibiotics, culture conditions, and even the plasmid itself. This guide showcases how high your yield can be through simple steps.
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关键词: VSV immunotherapy, oncolytic virus, pseudotyping
Vesicular stomatitis virus (VSV), known for its simplicity and well-explored biology, serves as an invaluable model in virology, particularly through pseudotyping, where its envelope protein is swapped with that of another virus.
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关键词: Viral gene therapy, retrovirus, transient transfection
A comprehensive guide to selecting the appropriate viral vector for gene delivery experiments. The article examines how factors such as cell type, expression type, and insert size can impact your experiment while comparing the integration capabilities, carrying capacities, and tropisms of different viruses.
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关键词: Zolgensma mechanism, spinal muscular atrophy
Learn about spinal muscular atrophy from a genetic perspective and the mechanisms behind two key gene therapy treatments, Spinraza and Zolgensma.
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关键词: Viral titer, gene toxicity
If your viral titration is low, it could be because of titration method, improper vector or experimental design, or expression of toxic genes. Here we discuss troubleshooting all of these and more.
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关键词: AAV packaging, AAV capsid engineering, AAV serotype tropism
Dive into the complexities of choosing the right AAV serotype for gene delivery, this guide elucidates the genetic and structural differences between serotypes, their tissue specificity, and evolution, ultimately guiding researchers in selecting the most suitable serotype for their experiments.
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关键词: Viral vector, origin of replication, promoter
Learn about the components of a plasmid necessary for gene expression, including promoters, ORFs, polyA signals, selection markers, origins of replication, and antibiotic resistance genes.
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关键词: Zynteglo gene therapy, beta thalassemia
Learn about the genetic blood disorder beta thalassemia and the lentiviral and CRISPR/Cas9-based gene therapy approaches for treatment.
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关键词: Agarose gel electrophoresis, DNA conformation, ethidium bromide
A guide to how gel electrophoresis results are influenced by DNA conformation, ethidium bromide concentration, and method of introduction.
阅读更多 ››关键词: PCR, restriction enzymes, ligase
Dive deep into the different steps, strategies, and tricks of gene cloning including different vector backbones, plasmids, PCR, insert types, and gene delivery that are highly adaptable for multiple research scenarios.
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关键词: mRNA structure, lipid nanoparticles, mRNA vaccine
A guide that explores the structure of mRNA molecules, highlighting key features such as the 5' cap, untranslated regions, and coding sequence, and discusses how mRNA therapeutics leverage modified nucleotides and lipid nanoparticles for effective delivery.
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关键词: PCR primer, PCR template, PCR troubleshooting
An overview of PCR with tips on the template to use for your application and a guide to designing and setting up your reaction.
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关键词: Muscular dystrophy, gutless adenovirus, gene therapy
Learn about exciting gene therapy approaches utilizing AAV, adenovirus and gutless adenovirus for treating Duchenne muscular dystrophy.
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