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关键词: CRISPR library screen, in vitro and in vivo screens, antibiotic kill curve, lentivirus functional titration
Explore the critical steps in preparing and performing CRISPR screens and learn what to consider to set your screening project up for success.
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关键词: AAV, in vivo viral vector, therapeutic development, serotype tropism
Learn about AAV and the properties that have made it one of the most popular viral vectors for in vivo applications and therapeutic development.
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关键词: CRISPR screening, gRNA library design, in vitro and in vivo models, library preparation
Explore the critical steps in designing and performing CRISPR screens and learn what to consider to set your screening project up for success.
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关键词: Gene delivery seminar series, iPSCs, neurodegenerative disease, CRISPR
Dr. Narayan's innovative work shows how patient-derived iPSC models are transforming our understanding of AD while paving the way toward targeted therapies.
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关键词: CRISPR delivery, plasmid, virus, mRNA, RNP
A comparison of delivery systems to help you choose the optimal method for your CRISPR experiments, from research applications to clinical use.
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关键词: Lentivirus, Vector Design, Repetitive Sequences
Using two identical promoters in a lentiviral vector causes detrimental effects on proper expression of the two open reading frames.
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关键词: Promoter, cloning, CMV, CAG, lentivirus, AAV
This article highlights the role of ubiquitous promoters in gene delivery, discussing the advantages and limitations of viral and non-viral promoters, including CMV, CAG, and EF1α, to guide you in your vector design decisions.
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关键词: Cre recombinase, conditional knockout, Cre-lox system
The Cre-Lox system, featuring Cre recombinase and loxP sites, is a cornerstone of genetic engineering, enabling precise control over gene expression and modification.
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关键词: Transposable elements, transposase, PB transposon
Learn about optimization strategies gene delivery systems using transposons and transposases. Here we compare different systems like PB transposon, Sleeping Beauty, and Tol2, detailing their integration mechanisms, carrying capacities, and target preferences.
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关键词: AAV packaging, AAV capsid engineering, AAV serotype tropism
Dive into the complexities of choosing the right AAV serotype for gene delivery, this guide elucidates the genetic and structural differences between serotypes, their tissue specificity, and evolution, ultimately guiding researchers in selecting the most suitable serotype for their experiments.
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关键词: Viral vector, origin of replication, promoter
Learn about the components of a plasmid necessary for gene expression, including promoters, ORFs, polyA signals, selection markers, origins of replication, and antibiotic resistance genes.
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关键词: mRNA structure, lipid nanoparticles, mRNA vaccine
A guide that explores the structure of mRNA molecules, highlighting key features such as the 5' cap, untranslated regions, and coding sequence, and discusses how mRNA therapeutics leverage modified nucleotides and lipid nanoparticles for effective delivery.
阅读更多 ››关键词: PCR, restriction enzymes, ligase
Dive deep into the different steps, strategies, and tricks of gene cloning including different vector backbones, plasmids, PCR, insert types, and gene delivery that are highly adaptable for multiple research scenarios.
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