服务商工作站 >>
荧光报告基因细胞稳转株
云舟生物提供多种单克隆来源的报告基因细胞株，这些细胞株具有强劲稳定的荧光信号，是用于荧光成像、流式细胞术和药筛实验等多种研究应用的理想选择。.
订购信息
运输与保存
我们的现货细胞稳转株产品存储于含有10% DMSO的培养基，并以干冰形式运输。收到细胞时，请立即将冻存管转移到液氮蒸汽相中以长期保存。您也可以将细胞放置于-80℃以短期保存（≤1周）。
试验验证
- EGFP表达HT1080细胞
- EGFP表达293T细胞
- EGFP/mCherry双荧光表达293T细胞
图1 EGFP表达HT1080细胞的典型荧光图。放大倍数：100x。
图2 EGFP表达293T细胞的典型荧光图。放大倍数：100x。
图3 EGFP/mCherry双荧光表达293T细胞的典型荧光图。放大倍数：100x。
精选文献
A lysine-rich cluster in the N-BAR domain of ARF GTPase-activating protein ASAP1 is necessary for binding and bundling actin filaments
Anjelika Gasilina, et al.
J Biol Chem, 2022, doi: 10.1016/j.jbc.2022.101700
使用的云舟生物产品或服务:Abstract: Actin filament maintenance is critical for both normal cell homeostasis and events associated with malignant transformation. The ADP-ribosylation factor GTPase-activating protein ASAP1 regulates the dynamics of filamentous actin-based structures, including stress fibers, focal adhesions, and circular dorsal ruffles. Here, we have examined the molecular basis for ASAP1 association with actin. Using a combination of structural modeling, mutagenesis, and in vitro and cell-based assays, we identify a putative-binding interface between the N-Bin-Amphiphysin-Rvs (BAR) domain of ASAP1 and actin filaments. We found that neutralization of charges and charge reversal at positions 75, 76, and 79 of ASAP1 reduced the binding of ASAP1 BAR-pleckstrin homology tandem to actin filaments and abrogated actin bundle formation in vitro. In addition, overexpression of actin-binding defective ASAP1 BAR-pleckstrin homology [K75, K76, K79] mutants prevented cellular actin remodeling in U2OS cells. Exogenous expression of [K75E, K76E, K79E] mutant of full-length ASAP1 did not rescue the reduction of cellular actin fibers consequent to knockdown of endogenous ASAP1. Taken together, our results support the hypothesis that the lysine-rich cluster in the N-BAR domain of ASAP1 is important for regulating actin filament organization.
>展开<收起
The KU-PARP14 axis differentially regulates DNA resection at stalled replication forks by MRE11 and EXO1
Ashna Dhoonmoon, Claudia M. Nicolae & George-Lucian Moldovan.
Nature Communications, 2022, doi: 10.1038/s41467-022-32756-5
使用的云舟生物产品或服务:Abstract: Suppression of nascent DNA degradation has emerged as an essential role of the BRCA pathway in genome protection.In BRCA-deficient cells, the MRE11 nuclease is responsible for both resection of reversed replication forks, and accumulation of single stranded DNA gaps behind forks. Here, we show that the mono-ADP-ribosyltransferase PARP14 is a critical co-factor of MRE11. PARP14 is recruited to nascent DNA upon replication stress in BRCA-deficient cells, and through its catalytic activity, mediates the engagement of MRE11. Loss or inhibition of PARP14 suppresses MRE11-mediated fork degradation and gap accumulation, and promotes genome stability and chemoresistance of BRCA-deficient cells. Moreover, we show that the KU complex binds reversed forks and protects them against EXO1-catalyzed degradation. KU recruits the PARP14-MRE11 complex, which initiates partial resection to release KU and allow long-range resection by EXO1. Our work identifies a multistep process of nascent DNA processing at stalled replication forks in BRCA-deficient cells.
>展开<收起文档
暂无
常见问题解答
云舟生物提供的所有现货细胞稳转株均来源于单克隆细胞株，以保证实验的一致性和可重复性。我们的单克隆细胞株经过严格的QC以及功能验证，可为您的下游应用提供充分的保障。
相关服务
细胞稳转株构建载体克隆
慢病毒
文库构建