灵光一闪时刻

Figure 1. Workflow of CRISPR-based knockout screens. Adapted from Acta Biochim Biophys Sin 44:103-112 (2012). To ensure that a CRISPR library screen is set up for success, several key considerations must be addressed from the very beginning. Part 1 of this blog post series will focus on the early stages of a library screening experiment, including selecting an appropriate biological system and designing a suitable library specific to your application....View more

灵光一闪时刻

A gel that is run at 100 V for about 1 hour will generally have large pieces of DNA that have travelled a short distance, and smaller pieces that have travelled a longer distance. Using a reference ladder with bands of known sizes, researchers can confirm that their DNA sample contains the appropriately sized DNA. Bands of DNA can then be excised and recovered for downstream applications. Figure 1....View more

灵光一闪时刻

GOI | Promoter Strength | Total Yield (vg) ---|---|--- mFoxn1:P2A:EGFP | #1 (strong) | 1.16x1011 mFoxn1:P2A:EGFP | #1 (strong) | 3.46x1011 mFoxn1:P2A:EGFP | #3 (medium strong) | 1.56x1012 EGFP | #3 (medium strong) | 2.05x1012 Additionally, inducible or tissue specific promoters can be utilized to keep transgene expression levels low in packaging cells....View more

灵光一闪时刻

A gel that is run at 100 V for about 1 hour will generally have large pieces of DNA that have travelled a short distance, and smaller pieces that have travelled a longer distance. Using a reference ladder with bands of known sizes, researchers can confirm that their DNA sample contains the appropriately sized DNA. Bands of DNA can then be excised and recovered for downstream applications. Figure 1. Set up of gel electrophoresis and visualization of results....View more