基因递送热点聚焦

Figure 1. Workflow of CRISPR-based knockout screens. Adapted from Acta Biochim Biophys Sin 44:103-112 (2012). To ensure that a CRISPR library screen is set up for success, several key considerations must be addressed from the very beginning. Part 1 of this blog post series will focus on the early stages of a library screening experiment, including selecting an appropriate biological system and designing a suitable library specific to your application....View more

基因递送热点聚焦

. ## Unraveling the Concept of Pseudotyping One standout application of VSV lies in pseudotyping, where the native G protein is swapped with an envelope protein from another virus. Pseudotyping hinges on a seemingly simple yet transformative process. By substituting the envelope protein of one virus with that of another, pseudotyped viral particles are born--bearing the genetic material of one virus and the surface characteristics of another....View more

基因递送热点聚焦

Figure 1. Key phases of drug development and their major challenges. The next phases of development (Figure 1) require optimizing drug efficacy and scalability while meeting regulatory guidelines and ensuring Good Manufacturing Practice (GMP)-compliant, consistent manufacturing. Since every therapeutic is unique, cell and gene therapy (CGT) development isn't cookie- cutter; each product requires tailored, rigorous process development for optimal results....View more