搜索“win11专where 一样送到菜鸟苷酸结合蛋白1”得到105个结果，以下为结果71-80
通常情况下，将感兴趣的增强子克隆到该载体中，并将构建好的载体用于制备转基因小鼠。对转基因胚胎或成年小鼠中LacZ报告基因的表达情况进行检测，可以进一步分析增强子的活性。
该载体系统可用于筛选增强子元件，分析增强子的组织特异性，比较不同增强子的活性，谱系追踪以及其他应用。
关于该载体系统的更多信息，请参考一下文献。
参考文献 | 主题
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Nature. 444:499-502 (2006) | Use of the vector systemtocarry out genome-wide testing of putative enhancers in the mouse
Development. 105:707-714 (1989) | Cloning of the Hsp68 minimal promoter
## 亮点
我们的载体系统是基于常规质粒基因表达载体而构建的。待测增强子位于Hsp68最小启动子（Hsp68_mini）的上游，可控制下游LacZ报道基因的表达。...
在细胞培养中使用该载体系统时，可将抗生素或荧光标记添加到载体中，使转染后的细胞可被筛选或可示踪以便于分离出已永久整合载体的细胞。
PB转座系统包含两个组分，一个组分为PBase转座酶（通常为表达PBase的IVT
mRNA）；另一个组分被称为转座子质粒，包含两个末端重复序列（TR）以及两者之间的被转座区域，需要被转座到宿主基因组中的目的基因就克隆在这个区域。
当表达PBase的IVT mRNA和转座子质粒共转染靶细胞时，PBase IVT
mRNA产生的转座酶将会识别转座子的两个TR元件，然后将被转座区和两个TR元件插入到宿主基因组中。转座插入通常发生在包含TTAA序列的宿主染色体位点，并在转座子两侧出现TTAA重复序列。
PB转座子属于II类转座子，通过"剪切--粘贴"的机制移动，从一个地方转座到另一个地方，而不留下序列本身（恰好相反，I类转座子是通过"复制--
粘贴"的方式移动）。由于辅助质粒是通过瞬时转染进入宿主细胞的，故会逐渐丢失。随着辅助质粒的丢失，转座子在宿主基因组中变成了永久整合。...
在细胞培养中使用该载体系统时，可将抗生素或荧光标记添加到载体中，使转染后的细胞可被筛选或可示踪以便于分离出已永久整合载体的细胞。
关于该载体系统和Cre介导重组的更多信息，请参考以下文献。...
It is then transfected into packaging cells along with helper
plasmids, where the region of the vector between the two inverted terminal
repeats (ITRs) is packaged into live virus. The donor and CRISPR target
sequences placed in-between the two ITRs are introduced into target cells
along with the rest of viral genome....
It is then transfected into packaging cells along with helper
plasmids, where the region of the vector between the two inverted terminal
repeats (ITRs) is packaged into live virus. The donor and CRISPR target
sequences placed in-between the two ITRs are introduced into target cells
along with the rest of viral genome....
It is then transfected into packaging
cells, where the region of the vector between the ITRs is packaged into live
virus. When the virus is added to target cells, the DNA cargo is delivered
into cells where it enters the nucleus and remains as episomal DNA without
integration into the host genome. The gRNA expression cassette placed in-
between the two ITRs during vector construction is introduced into target
cells along with the rest of viral genome....
Examples of such applications include: 1) paired Cas9 nickase
experiments where the "nickase" mutant form (hCas9-D10A) of hCas9 is used in
conjunction with two gRNAs targeting the two opposite strands of a single
target site to generate single strand cuts one on each strand, thereby leading
to a DSB with increased targeting specificity than a single gRNA; 2)
generating deletion of a fragment between two DSBs targeted by a pair of
gRNAs; and 3) targeting two different genes simultaneously....
Examples of such applications include: 1) paired Cas9 nickase
experiments where the "nickase" mutant form (hCas9-D10A) of hCas9 is used in
conjunction with two gRNAs targeting the two opposite strands of a single
target site to generate single strand cuts one on each strand, thereby leading
to a DSB with increased targeting specificity than a single gRNA; 2)
generating deletion of a fragment between two DSBs targeted by a pair of
gRNAs; and 3) targeting two different genes simultaneously....
It utilizes AAV-mediated delivery of a
polycistronic expression cassette consisting of one or more miR30-based shRNAs
(shRNAmiR) targeting gene(s) of interest and a user-selected ORF, where the
vector remains as episomal DNA without integration into the host genome. The
shRNAmiR transcript is processed by endogenous, cellular micro-RNA pathways to
produce mature shRNAs, which facilitate degradation of target gene mRNAs....
The adenovirus CRISPR vector is a highly efficient viral vehicle for
adenovirus-mediated introduction of both Cas9 and the target site-specific
gRNA sequence into a variety of mammalian cell types, where the vector remains
as episomal DNA without integration into the host genome. It is the preferred
gene delivery system in vivo, often used in gene therapy and vaccination.
An adenovirus CRISPR vector is first constructed as a plasmid in E. coli....
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