RNA干扰(RNA interference,简称RNAi)是一项强大的技术,能在多种细胞中有效地敲低基因表达水平,可用于研究基因功能。尽管RNAi的最简单方法是小干扰RNA(small interfering RNA,简称siRNA)寡核苷酸的胞质递送,但该技术仅限于能够转染的细胞,并且主要用于瞬时体外研究。通过慢病毒载体将短发夹RNA(short hairpin RNA,简称shRNA)引入哺乳动物细胞,可实现shRNA的稳定整合、靶基因的长期敲低,能感染分裂和非分裂细胞。
U6启动子驱动shRNA持续高水平表达,高效敲低靶基因
服务名称 | 交付内容 | 周期 |
---|---|---|
shRNA干扰慢病毒载体构建与慢病毒包装(3件装) |
| 15-28天 |
自定义Pol III启动子驱动表达shRNA,适合细胞或组织特异性敲低靶基因
服务名称 | 交付内容 | 周期 |
---|---|---|
miR30-shRNA干扰慢病毒载体构建与慢病毒包装(3件装) |
| 20-34天 |
shRNA非长期性表达,时间特异性调控基因干扰
服务名称 | 交付内容 | 周期 |
---|---|---|
IPTG诱导型shRNA干扰慢病毒载体构建与慢病毒包装(3件装) |
| 15-28天 |
慢病毒可以感染分裂与非分裂细胞,带来比质粒瞬转更良好的基因转导效果。慢病毒转基因稳定整合靶细胞基因组,细胞多次传代后仍然保持转基因的有效表达。
生产规格 | Unit Size | 滴度 | 分装 |
---|---|---|---|
慢病毒⼩量制备 | >2.5x107 TU | >1x108 TU/ml | 10x25 μl |
慢病毒中量制备 | >1x108 TU | >1x108 TU/ml | 10x100 μl |
慢病毒大量制备 | >1x109 TU | >1x109 TU/ml | 10x100 μl |
超纯化慢病毒中量制备 | >5x108 TU | >1x109 TU/ml | 10x50 μl |
超纯化慢病毒大量制备 | >1x109 TU | >1x109 TU/ml | 10x100 μl |
Feng, Fen et al. “LncPrep + 96kb 2.2 kb Inhibits Estradiol Secretion From Granulosa Cells by Inducing EDF1 Translocation.” Frontiers in cell and developmental biology vol. 8 481. 30 Jun. 2020.
Shows the effect of EDF1-specific shRNA on aromatase expression at the mRNA and protein levels. The experiment was independently repeated for three times. *p < 0.05, which was a specific two-group comparison.
Ichikawa, Akihiro et al. “Chaperone-mediated autophagy receptor modulates tumor growth and chemoresistance in non-small cell lung cancer.” Cancer science vol. 111,11 (2020): 4154-4165.
LAMP2A modulates lung cancer cell proliferation and chemoresistance in vitro. A, Western blot analysis of the expression levels of LAMP2A in A549 or H460 -shNC, -shLAMP2A#1, and #2 cells. B, Cell proliferation (%) of A549 or H460 -shNC, -shLAMP2A#1, and #2 cells. **P < .001 and ***P < .0001, by ANOVA and Bonferroni's post-test. E, Cell viability (%) was obtained by MTT with different doses of cisplatin (CDDP) or paclitaxel (PTX) in A549 or H460 -shNC,-shLAMP2A#1, and #2 cells.