元件指南

VectorBuilder提供了许多常用的载体元件，可供用户在设计载体时进行选择。下表按类型提供了这些常用元件的详细信息。

姓名	引导序列	Target Gene	Target Position	References	序列
Scramble[gRNA#1]	GTGTAGTTCGACCATTCGTG	None in human and mouse		Engineered by VectorBuilder	View
Scramble[gRNA#2]	GTTCAGGATCACGTTACCGC	None in human and mouse		Engineered by VectorBuilder	View
Scramble[SagRNA#1]	GTGTAGTTCGACCATTCGTG	None in human and mouse		Engineered by VectorBuilder	View
Scramble[SagRNA#2]	GTTCAGGATCACGTTACCGC	None in human and mouse		Engineered by VectorBuilder	View
Scramble[msgRNA#1]	GTGTAGTTCGACCATTCGTG	None in human and mouse		Engineered by VectorBuilder	View
Scramble[msgRNA#2]	GTTCAGGATCACGTTACCGC	None in human and mouse		Engineered by VectorBuilder	View
EGFP[gRNA#1]	GGGCGAGGAGCTGTTCACCG	EGFP	12-31	Science. 343:84 (2014)	View
EGFP[gRNA#2]	GAAGTTCGAGGGCGACACCC	EGFP	339-358	Science. 343:84 (2014)	View
ROSA26[gRNA#1]	CGCCCATCTTCTAGAAAGAC	Mouse ROSA26 locus	1st intron	Designed by VectorBuilder	View
AAVS1[gRNA#1]	GGGGCCACTAGGGACAGGAT	Human AAVS1 locus	1st intron	Science. 339:823 (2013)	View
EMX1[SagRNA#1]	GGCCTCCCCAAAGCCTGGCCA	Human EMX1	3rd exon	Engineered by VectorBuilder	View
Scramble[msSagRNA#1]	GTGTAGTTCGACCATTCGTG	None in human and mouse		Engineered by VectorBuilder	View
Scramble[msSagRNA#2]	GTTCAGGATCACGTTACCGC	None in human and mouse		Engineered by VectorBuilder	View
ttTi5605[gRNA#1]	GATATCAGTCTGTTTCGTAA	C. elegans ttTi5605 locus	Adjacent to the ttTi5605 Mos1 insertion site on chromosome II	Nat Methods. 10:1028 (2013)	View