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元件指南
VectorBuilder提供了许多常用的载体元件，可供用户在设计载体时进行选择。下表按类型提供了这些常用元件的详细信息。
Selection Marker Cassettes
| 姓名 | 描述 | Application Notes | Selection Drug | References | 序列 |
|---|---|---|---|---|---|
| Neo-cassette | mPGK promoter driving Neo with SV40 late pA | Allows cells to be resistant to geneticin (G418). | Geneticin (G418) | Engineered by VectorBuilder | View |
| Puro-cassette | mPGK promoter driving Puro with SV40 late pA | Allows cells to be resistant to puromycin. | Puromycin | Engineered by VectorBuilder | View |
| LoxP-Neo-LoxP | mPGK promoter driving Neo with SV40 late pA, flanked by LoxP | Allows cells to be resistant to geneticin (G418); can be deleted by Cre recombinase. | Geneticin (G418) | Engineered by VectorBuilder | View |
| FRT-Neo-FRT | mPGK promoter driving Neo with SV40 late pA, flanked by FRT | Allows cells to be resistant to geneticin (G418); can be deleted Flp recombinase. | Geneticin (G418) | Engineered by VectorBuilder | View |
| LoxP-Puro-LoxP | mPGK promoter driving Puro with SV40 late pA, flanked by LoxP | Allows cells to be resistant to puromycin; can be deleted by Cre recombinase. | Puromycin | Engineered by VectorBuilder | View |
| FRT-Puro-FRT | mPGK promoter driving Puro with SV40 late pA, flanked by FRT | Allows cells to be resistant to puromycin; can be deleted by Flp recombinase. | Puromycin | Engineered by VectorBuilder | View |
| 3xP3-DsRed | DsRed fluorescent marker driven by 3xP3 promoter | Used for identification of genetically engineered fly lines. | - | Insect Biochem Mol Biol. 32:1221 (2002) | View |
