元件指南

VectorBuilder提供了许多常用的载体元件，可供用户在设计载体时进行选择。下表按类型提供了这些常用元件的详细信息。
Selection Marker Cassettes
姓名描述Application NotesSelection DrugReferences序列
Neo-cassettemPGK promoter driving Neo with SV40 late pAAllows cells to be resistant to geneticin (G418).Geneticin (G418)Engineered by VectorBuilder View
Puro-cassettemPGK promoter driving Puro with SV40 late pAAllows cells to be resistant to puromycin.PuromycinEngineered by VectorBuilder View
LoxP-Neo-LoxPmPGK promoter driving Neo with SV40 late pA, flanked by LoxPAllows cells to be resistant to geneticin (G418); can be deleted by Cre recombinase.Geneticin (G418)Engineered by VectorBuilder View
FRT-Neo-FRTmPGK promoter driving Neo with SV40 late pA, flanked by FRTAllows cells to be resistant to geneticin (G418); can be deleted Flp recombinase.Geneticin (G418)Engineered by VectorBuilder View
LoxP-Puro-LoxPmPGK promoter driving Puro with SV40 late pA, flanked by LoxPAllows cells to be resistant to puromycin; can be deleted by Cre recombinase.PuromycinEngineered by VectorBuilder View
FRT-Puro-FRTmPGK promoter driving Puro with SV40 late pA, flanked by FRTAllows cells to be resistant to puromycin; can be deleted by Flp recombinase.PuromycinEngineered by VectorBuilder View
3xP3-DsRedDsRed fluorescent marker driven by 3xP3 promoterUsed for identification of genetically engineered fly lines.-Insect Biochem Mol Biol. 32:1221 (2002) View