元件指南
VectorBuilder提供了许多常用的载体元件,可供用户在设计载体时进行选择。下表按类型提供了这些常用元件的详细信息。
常用gRNA
姓名 | 引导序列 | Target Gene | Target Position | References | 序列 |
---|---|---|---|---|---|
Scramble[gRNA#1] | GTGTAGTTCGACCATTCGTG | None in human and mouse | Engineered by VectorBuilder | View | |
Scramble[gRNA#2] | GTTCAGGATCACGTTACCGC | None in human and mouse | Engineered by VectorBuilder | View | |
Scramble[SagRNA#1] | GTGTAGTTCGACCATTCGTG | None in human and mouse | Engineered by VectorBuilder | View | |
Scramble[SagRNA#2] | GTTCAGGATCACGTTACCGC | None in human and mouse | Engineered by VectorBuilder | View | |
Scramble[msgRNA#1] | GTGTAGTTCGACCATTCGTG | None in human and mouse | Engineered by VectorBuilder | View | |
Scramble[msgRNA#2] | GTTCAGGATCACGTTACCGC | None in human and mouse | Engineered by VectorBuilder | View | |
EGFP[gRNA#1] | GGGCGAGGAGCTGTTCACCG | EGFP | 12-31 | Science. 343:84 (2014) | View |
EGFP[gRNA#2] | GAAGTTCGAGGGCGACACCC | EGFP | 339-358 | Science. 343:84 (2014) | View |
ROSA26[gRNA#1] | CGCCCATCTTCTAGAAAGAC | Mouse ROSA26 locus | 1st intron | Designed by VectorBuilder | View |
AAVS1[gRNA#1] | GGGGCCACTAGGGACAGGAT | human AAVS1 locus | Human AAVS1 locus | Science. 339:823 (2013) | View |
EMX1[SagRNA#1] | GGCCTCCCCAAAGCCTGGCCA | Human EMX1 | 3rd exon | Engineered by VectorBuilder | View |
Scramble[msSagRNA#1] | GTGTAGTTCGACCATTCGTG | None in human and mouse | Engineered by VectorBuilder | View | |
Scramble[msSagRNA#2] | GTTCAGGATCACGTTACCGC | None in human and mouse | Engineered by VectorBuilder | View | |
ttTi5605[gRNA#1] | GATATCAGTCTGTTTCGTAA | C. elegans ttTi5605 locus | Adjacent to the ttTi5605 Mos1 insertion site on chromosome II | Nat Methods. 10:1028 (2013) | View |