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向使用过VectorBuilder产品和服务的客户表示感激。你们的满意,才是我们鞠躬尽瘁的动力。在此,很荣幸分享你们的感谢信。谢谢!

  • I have been working with VectorBuilder from the beginning of my postdoc. I’ve chosen VectorBuilder because of the online platform to create customized vectors. It is really impressive how easy is to digitally clone and the order process. Specifically, in our paper, we’ve used VectorBuilder for several critical experiments, including the downregulation of Bassoon protein (AAV-shRNA) and to study the role of Bassoon in the propagation of tau (AAV-GFP-P2A-hTau). These experiments were extremely important to probe our hypothesis.

    Dr. Pablo A. Martínez

    Indiana University School of Medicine, USA

  • It has been a great experience working with VB. I was one of the first to test their empty capsid (AAV VLP) service. For my batch capsids were nearly completely empty (>97% by AUC and TEM) and were thus a huge help for assay development as a negative control. I also want to highlight that my contact Matthew Wheeler was always very helpful and transparent for any queries I had and that the team is very agile. A big plus is also the very intuitive website. Overall, a big thumbs up for VectorBuilder from my side.

    Dr. Andrei Hutanu

    Analytical Biochemist & QC Method Development, Roche, Switzerland

  • 与云舟生物的合作相当棒!mRNA作为当今的热门疫苗技术,大量被用于多种传染性和非传染性疾病的预防和治疗。云舟生物的Daniel Meng研发团队为我快速搭建了一个IVT mRNA CRO平台,通过与他们的紧密合作我们共同开发了针对HIV和流感的mRNA疫苗。他们的科学专业性与问题解决能力让我印象深刻。云舟生物再一次向我证明他们是值得信赖的合作伙伴。

    Dr. Caijun Sun

    中山大学

  • Our laboratory has had great success with both viral constructs and ready-to-use virus prepared by VectorBuilder. They provide excellent customer service at a reasonable price, and their website is extremely user-friendly. Very pleased we discovered this company.

    Marco Seandel

    Weill Cornell Medical College, USA

  • VectorBuilder has provided incredible value and customer service throughout the entire process from product selection, custom design assistance, order submission, status reports and ultimate delivery management. The custom products I purchased from them performed exactly as advertised and in fact surpassed my expectations. I would recommend VectorBuilder to anyone requiring this type of product.

    Frank Borriello

    Alloplex Biotherapeutics, USA

  • We ordered adenovirus from VectorBuilder to overexpress our protein of interest in primary mouse hepatocytes. The ordering process, including vector design, was very fast and easy. We received the plasmid and adenovirus within the time frame that was stated during the ordering process and the transduction efficiency of the adenovirus was very high, enabling us to use a low MOI. As a bonus, we also received the control Ad-EGFP adenovirus for free. In the future, I will not use any more siRNA or classical transfection methods on primary mouse hepatocytes, just adenoviruses from VB! I can highly recommend VectorBuilder's services for vector design and adenovirus production.

    Alexander Mayer

    University of Würzburg, Germany

  • VectorBuilder is an intuitive tool to help your need in developing vectors at an affordable price. I was in need of a CRISPR Lentiviral system with a Puro selection due to working with delicate cells that cannot handle FACS sorting pressures. With their online interface, it was easy to construct the vector I wanted and received the vector in a timely manner! It was simple to produce virus and test it in our cell culture and had good knockdown clones. I definitely will be ordering more vectors from Cyagen to finish up my thesis!

    Christophe Langouet-Astrie

    University of Colorado, Denver

  • Amazing experience with VectorBuilder! Ordered plasmids beginning of December, and despite the holiday season they arrived exactly as scheduled. Furthermore, customer support reached out to ask what would be my desired delivery date, considering potential holiday time at my institute. Overall, I am super satisfied, would highly recommend VectorBuilder (which I already do) and will buy from them again. All the best from my side!

    Tamara Tomin

    Medical University of Graz, Austria

  • We began to use VectorBuilder to prepare adenoviral plasmids about a year ago. We normally handled all the cloning stuffs ourselves, but when it comes to adenoviral vectors, because of its large size, sometimes it is kind of tricky for inexperienced students. We decided to give a try on VectorBuilder. One month later, the plasmid arrived and it generated virus that expressed the correct gene. After that, we have ordered more than 10 adenoviral plasmids and they all work fine. VectorBuilder did save us a lot of time.

    Lei Sun

    DUKE-NUS Graduate Medical School, Singapore

  • I ordered a plasmid vector two years ago. The design on the web platform was easy. Contacts with the specialists to arrange further details were excellent: they are very supportive!

    Emanuela Signori

    Consiglio Nazionale delle Ricerche, Italia

  • I have been using VectorBuilder's cloning service for several years, and am extremely happy with their products and service. Their online design software is very easy to use and provides the most cutting edge solutions for today's most demanding molecular cloning needs. Their customer service is outstanding, with great technical competence and is always on time to delivery the excellent results, which have saved me months of precious research time. I would recommend VectorBuilder to anyone who want the best vector design and molecular cloning service.

    Guohao Dai

    Northeastern University, USA

  • We have ordered quite a few plasmids from VectorBuilder in the past 1-2 years, ranging from regular plasmids, piggybac, and AAV vectors. The products have been delivered on time and worked as expected. Highly recommend!

    Shu-Hsien Sheu

    Janelia Research Campus of Howard Hughes Medical Institute, USA

  • Using VectorBuilder has transformed the way I do my research. VectorBuilder’s easy-to-use web interface allows me to custom build my own plasmids, freeing up time that I would have wasted cloning and instead letting me focus on the research that really matters. They offer great prices with a fast turnaround time. Their friendly and well-informed technical support team are on hand every step of the way, should you need any help. I would strongly recommend VectorBuilder to anyone wanting to accelerate the progress of their research.

    David M Brown

    University of Nottingham, UK

  • The scientific standards of VectorBuilder’s services are unquestionably high. The web interface to design vectors is intuitive and in fact so easy to use that it can even serve for educational purposes. VectorBuilder has pretty much covered everything one can think about when wanting to design an expression vector, it’s great to have all the versatility on one page. Turnaround time is fast and owing to their professionalism and customer friendly attitude I can recommend VectorBuilder’s services without reserve.

    Johnny Kim

    Max Planck Institute for Heart and Lung Research, Germany

  • 我已经使用了云舟生物的病毒包装服务两年了。病毒的质量很好,我的项目也进行得很顺利。我在SCI上发表的2篇文章都使用了云舟生物的产品。

    Wei Zhang

    浙江大学

  • We recently purchased from VectorBuilder a lentiviral plasmid for constitutive expression of GFP and luciferase and the corresponding lentivirus. We had infected several tumor cell lines with excellent results. The lentiviral stocks worked very nice saving us a lot of time. We are now using these modified cell lines for monitoring tumor growth in animal models using in vivo imaging.

    Javier Alonso

    Research institute for Rare Diseases, Spain

  • VectorBuilder’s service makes designing vectors incredibly simple and there’s no software required, it can all be done through their easy to use website. The lentivirus production service is a massive bonus as it’s so quick and reasonably priced there’s really no reason to make our own lentivirus anymore. Thanks for a fantastic service.

    Timothy London

    TC Biopharm, UK

  • 很赞!对比了多家公司后我最终选择了云舟生物。他们提供的产品质量高、性价比强。

    Lihua Xu

    中山大学附属第一医院

  • VectorBuilder is an amazing way to save time on molecular biology. The web site is easy to use, well-conceived and allows quick design in most cases. For more unusual requests, the team is energetic, reply promptly on every request, and always finds a way to help. Low prices and short timelines really make it possible to consider VectorBuilder on many cloning matters.

    Laura Israel

    Novartis (Basel), Switzerland

  • 我使用过云舟生物的载体构建服务,也购买过他们的慢病毒。他们的病毒的交付周期很短,效果也很好。他们对客户的咨询反应也很快速。

    Xijuan Chen

    中山大学光华口腔医院

  • We requested a custom AAV (4-YF) from VectorBuilder. The ordering process was easy and the customer support team were really helpful. Our virus had the expected titer and worked extremely well for our chosen application.

    Jessica Rodgers

    The University of Manchester, UK

  • 我经常使用云舟生物的载体构建服务,对于他们的产品质量我总体很满意,我遇到的一些难题他们也能很快帮助我解决。

    Huimin Chen

    中山大学附属第一医院

  • VectorBuilder has provided very satisfactory service in making various AAV vector constructs for us. The new sales representative is very proactive in offering help on explaining and introducing other VectorBuilder service to us. Expecting to get more support and service from VectorBuilder.

    Qingchun Tong

    University of Texas Medical School at Ho, USA

  • I have been consistently using VectorBuilder over the last couple years and have been extremely satisfied with the vectors delivered to me. These include standard over-expression vectors, and Cas9 vectors on piggybac backbone. All of the vectors I have purchased so far have passed my QC and provided reproducible functional data.

    Daksh Thaper

    Vancouver Prostate Centre, Canada

  • I have been successfully cloning for 3+ years, but the last time we tried generating KO cell lines with CRISPR/Cas9 vectors, we were not successful. This was upsetting, especially since I invested a lot of time in these efforts. Then, by chance, I attended one of VectorBuilder's seminars. Due to their reasonable prices and the fact I could focus my time on other work, we decided to give their lentiviral (dual gRNA) Cas9 plasmid a try. The turnaround time was reasonable, their customer service was excellent and they actually gave feedback about the status of my cloning projects. Their website is also easy to use. But most importantly: using their Cas9 dual gRNA vector, I was able to successfully generate KO cell lines! 

    I recommend VectorBuilder to CRISPRCas9 users without any hesitation. 

    Anna Salamero Boix

    Georg-Speyer-Haus Frankfurt, Germany

  • We applied CRISPR cas9 nickase in our study. VectorBuilder helped us design the gRNAs and construct plasmids with double gRNAs and double selection markers (EGFP/puromycin). The gRNA specificity is quite good, better than available online design tools. We got the knockout cell lines in the first run of electroporation with no off-target events. I would recommend VectorBuilder to CRISPR cas9 users.

    Jie Qin

    University Clinic Cologne, Germany

  • VectorBuilder is an intuitive tool for developing vectors at affordable prices. I was in need of a CRISPR lentiviral system with a Puro selection cassette due to working with delicate cells that cannot handle FACS sorting pressures. With their online interface, it was easy to construct the vector I wanted and received the vector in a timely manner! It was simple to produce virus and test it in our cell culture and generated clonal cells with good gene knockdown. I definitely will be ordering more vectors from VectorBuilder to finish up my thesis!VectorBuilder is an intuitive tool for developing vectors at affordable prices. I was in need of a CRISPR lentiviral system with a Puro selection cassette due to working with delicate cells that cannot handle FACS sorting pressures. With their online interface, it was easy to construct the vector I wanted and received the vector in a timely manner! It was simple to produce virus and test it in our cell culture and generated clonal cells with good gene knockdown. I definitely will be ordering more vectors from VectorBuilder to finish up my thesis!

    Christophe Langouet-Astrie

    University of Colorado, USA

  • I recently met one of the VectorBuilder product specialist during AAI-2019 meeting at San Diego Convention Center. Her name is Dr. Swati Roy, and she provided me excellent information regarding their products and vector designs. And then, we recently purchased CRISPR Cas9 dual gRNA vector. Now, we are ordering few more plasmid vectors. As per my experiences, VectorBuilder customer services are excellent and user-friendly. The VectorBuilder product prices are reasonable.I recently met one of the VectorBuilder product specialist during AAI-2019 meeting at San Diego Convention Center. Her name is Dr. Swati Roy, and she provided me excellent information regarding their products and vector designs. And then, we recently purchased CRISPR Cas9 dual gRNA vector. Now, we are ordering few more plasmid vectors. As per my experiences, VectorBuilder customer services are excellent and user-friendly. The VectorBuilder product prices are reasonable.

    Shahanshah Khan

    UT Southwestern Medical Center, USA

  • 云舟生物为我的shRNA敲低实验提供了载体构建和慢病毒包装服务。他们的慢病毒的转导效率很高,而且员工也非常专业,总能很快回复我的技术问题。在云舟生物帮助下,我很快完成了我的实验,目前已经在准备论文投稿的工作。

    Mengyuan Fan

    北京林业大学自然保护学院

  • We have used VectorBuilder for multiple vectors including regular, lentiviral, Tet-inducible, and CRISPR/Cas9 vectors. Designing the vectors is super easy and we have had good luck with them. I would definitely recommend their products!

    Steven Wall

    Texas A&M University, USA

  • Vector Builder was fast, efficient and easy to use. We received our viral vector promptly and AAV9 has performed exactly how'd we hoped. Thank you!

    Alexander Alimov

    University of Kentucky, USA

  • Before using VectorBuilder, producing lentiviral overexpression vectors for our in vitro models was a recurring and time-consuming enterprise. We faced frequent challenges in every experimental step, which made it difficult to plan the actual experiments. Setting up the process with VectorBuilder was very efficient, thanks to their great expertise and support. Once we started using VectorBuilder's services, we noticed a substantial increase in productivity as well as a more uniform and predictable transduction pattern of our target cells.

    We went on to use VectorBuilder for a large-scale CRISPR screen with a custom library spanning over 1,600 genes. With the provided virus, we were able to easily maintain cell representation and we derived a number of interesting hit genes, which we are excited to follow up on. A big thank you to the entire VectorBuilder Team!

    Dominic Schmid

    University of Basel

  • We recently purchased a lentiviral plasmid for constitutive expression of GFP and luciferase and the corresponding lentivirus from VectorBuilder. We have infected several tumor cell lines with excellent results. The lentiviral stocks worked very nicely, saving us a lot of time. We are now using these modified cell lines for monitoring tumor growth in animal models using in vivo imaging.

    Javier Alonso

    Research institute for Rare Diseases, Spain

  • 我购买过云舟生物的慢病毒。云舟生物的慢病毒滴度有保证,转导后荧光也很强。对于基因敲低的效率我也很满意。

    Yunhao Liang

    南方医科大学

  • I have been using ready-to-use lentiviruses from Vectorbuilder and they are working great. I used them against iPSC-derived neurons, and the transduction and expression of transgenes are remarkable. The vector backbones provide multiple options to tag your GOIs with fluorescent proteins or implement antibiotic selection markers. Their vector designing tools are helpful. Customer service is reliable. Good luck!

    Utpal Das

    University of California San Diego, USA

  • I used the lentivirus packaging service from VectorBuilder, and the process is very easy. All I needed to do is to ship a small quantity of transfer plasmid, and VectorBuilder will do the rest. Then I received the high titer virus at the end.

    Xueqing Xia

    UT MD Anderson Cancer Center, USA

  • We were searching for a way to outsource the cloning of our vectors and we are very happy with the service. The versatility of the tools make it a great time saver and the vectors were delivered on-time. Lentiviruses packaged are also very reliable and have been very successful in transduction studies in primary cells. Great customer service with fast response times and the team at VectorBuilder are very knowledgeable.

    Kathy Ngo

    Aridis Pharmaceuticals, USA

  • We ordered adenovirus from Vectorbuilder company to overexpress our interest genes. The service is fast, considerate, and high-qualified. Besides, the adenovirus is very effective to overexpress our target gene.

    Wanbao Yang

    Texas A&M University, USA

  • We ordered adenovirus from VectorBuilder to overexpress our protein of interest in primary mouse hepatocytes. The ordering process, including vector design, was very fast and easy. We received the plasmid and adenovirus within the time frame that was stated during the ordering process and the transduction efficiency of the adenovirus was very high, enabling us to use a low MOI. As a bonus, we also received the control Ad-EGFP adenovirus for free. In the future, I will not use any more siRNA or classical transfection methods on primary mouse hepatocytes, just adenoviruses from VB! I can highly recommend VectorBuilder's services for vector design and adenovirus production.

    Alexander Mayer

    University of Würzburg, Germany

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