VectorBuilder is an intuitive tool to help your need in developing vectors at an affordable price. I was in need of a CRISPR Lentiviral system with a Puro selection due to working with delicate cells that cannot handle FACS sorting pressures. With their online interface, it was easy to construct the vector I wanted and received the vector in a timely manner! It was simple to produce virus and test it in our cell culture and had good knockdown clones. I definitely will be ordering more vectors from Cyagen to finish up my thesis!
Christophe Langouet-Astrie
University of Colorado, Denver
I have been consistently using VectorBuilder over the last couple years and have been extremely satisfied with the vectors delivered to me. These include standard over-expression vectors, and Cas9 vectors on piggybac backbone. All of the vectors I have purchased so far have passed my QC and provided reproducible functional data.
Daksh Thaper
Vancouver Prostate Centre, Canada
I have been successfully cloning for 3+ years, but the last time we tried generating KO cell lines with CRISPR/Cas9 vectors, we were not successful. This was upsetting, especially since I invested a lot of time in these efforts. Then, by chance, I attended one of VectorBuilder's seminars. Due to their reasonable prices and the fact I could focus my time on other work, we decided to give their lentiviral (dual gRNA) Cas9 plasmid a try. The turnaround time was reasonable, their customer service was excellent and they actually gave feedback about the status of my cloning projects. Their website is also easy to use. But most importantly: using their Cas9 dual
I recommend VectorBuilder to CRISPRCas9 users without any hesitation.
Anna Salamero Boix
Georg-Speyer-Haus Frankfurt, Germany
We applied
Jie Qin
University Clinic Cologne, Germany
VectorBuilder is an intuitive tool for developing vectors at affordable prices. I was in need of a CRISPR lentiviral system with a Puro selection cassette due to working with delicate cells that cannot handle FACS sorting pressures. With their online interface, it was easy to construct the vector I wanted and received the vector in a timely manner! It was simple to produce virus and test it in our cell culture and generated clonal cells with good gene knockdown. I definitely will be ordering more vectors from VectorBuilder to finish up my thesis!VectorBuilder is an intuitive tool for developing vectors at affordable prices. I was in need of a CRISPR lentiviral system with a Puro selection cassette due to working with delicate cells that cannot handle FACS sorting pressures. With their online interface, it was easy to construct the vector I wanted and received the vector in a timely manner! It was simple to produce virus and test it in our cell culture and generated clonal cells with good gene knockdown. I definitely will be ordering more vectors from VectorBuilder to finish up my thesis!
Christophe Langouet-Astrie
University of Colorado, USA
I recently met one of the VectorBuilder product specialist during AAI-2019 meeting at San Diego Convention Center. Her name is Dr. Swati Roy, and she provided me excellent information regarding their products and vector designs. And then, we recently purchased CRISPR Cas9 dual gRNA vector. Now, we are ordering few more plasmid vectors. As per my experiences, VectorBuilder customer services are excellent and user-friendly. The VectorBuilder product prices are reasonable.I recently met one of the VectorBuilder product specialist during AAI-2019 meeting at San Diego Convention Center. Her name is Dr. Swati Roy, and she provided me excellent information regarding their products and vector designs. And then, we recently purchased CRISPR Cas9 dual gRNA vector. Now, we are ordering few more plasmid vectors. As per my experiences, VectorBuilder customer services are excellent and user-friendly. The VectorBuilder product prices are reasonable.
Shahanshah Khan
UT Southwestern Medical Center, USA
Before using VectorBuilder, producing lentiviral overexpression vectors for our in vitro models was a recurring and time-consuming enterprise. We faced frequent challenges in every experimental step, which made it difficult to plan the actual experiments. Setting up the process with VectorBuilder was very efficient, thanks to their great expertise and support. Once we started using VectorBuilder's services, we noticed a substantial increase in productivity as well as a more uniform and predictable transduction pattern of our target cells.
We went on to use VectorBuilder for a large-scale CRISPR screen with a custom library spanning over 1,600 genes. With the provided virus, we were able to easily maintain cell representation and we derived a number of interesting hit genes, which we are excited to follow up on. A big thank you to the entire VectorBuilder Team!
Dominic Schmid
University of Basel
Lis R et al.
Nature. 545: 439 (2017)
Conversion of adult endothelium to immunocompetent haematopoietic stem cells.
Thomas CA et al.
Cell Stem Cell. 21: 319 (2017)
Modeling of TREX1-Dependent Autoimmune Disease using Human Stem Cells Highlights L1 Accumulation as a Source of Neuroinflammation.