Before using VectorBuilder, producing lentiviral overexpression vectors for our in vitro models was a recurring and time-consuming enterprise. We faced frequent challenges in every experimental step, which made it difficult to plan the actual experiments. Setting up the process with VectorBuilder was very efficient, thanks to their great expertise and support. Once we started using VectorBuilder's services, we noticed a substantial increase in productivity as well as a more uniform and predictable transduction pattern of our target cells.
We went on to use VectorBuilder for a large-scale CRISPR screen with a custom library spanning over 1,600 genes. With the provided virus, we were able to easily maintain cell representation and we derived a number of interesting hit genes, which we are excited to follow up on. A big thank you to the entire VectorBuilder Team!
University of Basel
Lis R et al.
Nature. 545: 439 （2017）
Conversion of adult endothelium to immunocompetent haematopoietic stem cells.
Thomas CA et al.
Cell Stem Cell. 21: 319 （2017）
Modeling of TREX1-Dependent Autoimmune Disease using Human Stem Cells Highlights L1 Accumulation as a Source of Neuroinflammation.